Drs. Koudinov
FASEB Journal 2001 article gateway

"A very nice manuscript... 
It deals with an important concept that is gaining definite momentum in the field of Alzheimer's disease research... 
The study is well designed and most of the control experiments have been performed as expected".
Anonymous FASEB J referee
FASEB J editor reply to authors, 22 March 2001


Essential Role for Cholesterol in Synaptic Plasticity and Neuronal Degeneration.
Koudinov AR, Koudinova NV
FASEB J (2001) Published online June 27, 2001. 10.1096/fj.00-0815fje

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To have brief condensed account of this full size research communication please read the article FASEB J Express Summary (present both online and in the hard copy of The FASEB Journal  August 2001 issue)

Lay language summary for this article is also available in the format of The Society for Neuroscience 2001 Press book article


There is no understanding of the role of cholesterol and phospholipids in the mechanisms of synaptic function and neurodegeneration. Here we report that cholesterol disbalance is critical for synaptic transmission and plasticity as investigated by a study of paired pulse facilitation (PPF) and long-term potentiation (LTP). Extracellular recording of field evoked postsynaptic potentials showed enhanced PPF ratio and an impairment of LTP in CA1 subfield of adult rat ex-vivo hippocampal slices subjected to cyclodextrin- or normal human CSF-HDL3-mediated cholesterol efflux. Immunofluorescence with antibodies against neurofilament and tau revealed that cholesterol and phospholipids depletion causes alteration of normal hippocampal neurites and appearance of PHF-tau in the mossy fibers. We further find that LTP and amyloid beta protein increase [14C]acetate label incorporation into newly synthesized hippocampal membrane lipids. Our results indicate importance of neuronal cholesterol redistribution and synthesis for synaptic plasticity and neurodegeneration.

Authors key words:
Amyloid beta protein, Alzheimer’s disease, Down’s syndrome, cholesterol and phospholipid efflux and synthesis, hippocampal slices, Niemann-Pick type C disease, tau phosphorylation

First preliminary account: Neurosci Lett 2000 S55: S30Read the abstract and/or order full presentation

(Also see Koudinovs SFN'2001 Meeting Central)

Postpublication account:  Science 2002 295: 2213

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